It has been known that bacterial staining is one of the fundamental procedures done when identifying microorganisms, hence, learning about it and obtaining skills to perform it has been given great importance. This activity led to the constant exposure of the students and researchers, alike, to the chemicals used to achieve results. The chemicals used, or in this case, the dyes, were synthetic in nature, thus they pose possible danger to those who are continuously using it. The purpose of this study is to determine the possibility of karonda (C. carandas) fruit and blue ternate (C. ternatea) flower crude extracts as alternative bacterial stains by observing their staining intensities on S. aureus. Methylene blue served as the positive control. Tannic acid was added as a mordant to the crude extracts and staining intensity was based on the Likert scale. Results showed that blue ternate flower had a mean score of 0.8 (low staining intensity), blue ternate with tannic acid had a mean score of 1.8 (average staining intensity) while karonda fruit crude extract had a mean score of 1.0 (low staining intensity), karonda with tannic acid had 1.2 (average staining intensity) compared to the control group which had a mean score of 3.0 (very high staining intensity. This means that the staining intensity of the crude extracts obtained from natural sources were way beyond the staining intensity of methylene blue which is of synthetic origin....

Staphylococcus aureus the leading cause of bacterial infections in hospitals and communities such as abscesses (boils), furuncles, and cellulitis is a major pathogen of increasing importance due to the rise in antibiotic resistance. Cardiospermum halicacabum a well-known plant was investigated to explore its capacity against bacterial activity such as S. aureus that would be useful in the production of compounds that can inhibit other pathogenic bacteria and other pathogenic microorganisms. This study attempted to extend such concept and explore the possibility of using halicacabum as possible agent in controlling human pathogens specifically the aureus. The mean (M) was used in order to determine the average zone of inhibition of aureus applied with balloon vine (C. halicacabum) leaf, fruit, and combined extracts, Amoxicillin (Positive control), and Ethanol (Negative control). Using the disc diffusion method, the crude extract of C. halicacabum showed that the greatest zone of inhibition of S. aureus was observed among those applied with Amoxicillin (M= 52mm), followed by those applied with balloon vine fruit extract (M=44mm) Both leaf extract (M=31mm and the combined leaf and fruit extract (M=30mm) also significantly exhibited inhibition against the S. aureus. There is no zone of inhibition observed among those applied with Ethanol (M=.00mm). The One-Way ANOVA test result shows that the p-value is .000 which is deemed to be statistically significant if p<.001....