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Medicinal and phytochemical properties of selected herbal plants in Central China and Western Visayas, Philippines
(United Federation of Fil-Am Educators, Inc., 2021-11)
This investigation was conducted to evaluate the medicinal and phytochemical properties of four selected herbal plants: Andrographis paniculata, Cassia obtusifolia, Leonurus japonicus and Pueraria lobata found in both Central China and Western Visayas, Philippines. Mixed method research design was employed, the quantitative on herbals' descriptions and phytochemical analysis, while qualitative on interview of traditional healers. The herbals were identified and authenticated by the research team. The phytochemical analyses used the methods: Extraction of plant parts, Thin Layer Chromatography, UV identification and High-Performance Liquid Chromatography. The bioactive compounds diterpenoid dehydroangropholide in A. paniculata supports the claim of local healers to treat stomach ache and diabetes; the terpenoid orange cassia and chrysophanol in C. obtusifolia seeds supports the treatment for ulcer, hepatitis or liver associated ailment, ringworm, scabies and psoriasis; the alkaloid leonurine hydrochloride in L. japonicus leaves supports the healing practices in treating menstrual disorders and fever; and the flavonoid puerarin in P. lobata tubers supports the claim of traditional healers in regulating menstruation, diuresis, fever and promotes blood circulation. Most healing practices in both study areas have scientific explanations....
Potential alpha-amylase and alpha-glucosidase inhibitory activity of bioactive fractions from alugbati (Basella alba) leaves
(2022-06)
This study evaluated the potential in vitro inhibitory activity of Basella alba leaves against enzyme markers of diabetes mellitus, α-amylase, and α-glucosidase. The dried plant sample was ground into fine powder, defatted, and soaked in methanol to obtain the crude extract. The crude methanolic extract of Basella alba was then fractionated sequentially in acetone-methanol mixtures with increasing solvent polarity ratios. Fractions obtained were evaluated for their α-amylase and α-glucosidase inhibitory potential. The standard drug, acarbose inhibited 37.44 ± 7.65%, 83.99 ± 0.86%, and 86.52 ± 0.40% of α-amylase activity at 10, 30, and 50 ppm. The highest inhibition was observed in the acetone-methanol (4:1) fraction which inhibited 57.80 ± 0.71%, 56.39 ± 0.49%, and 46.94 ± 0.26% of α-amylase activity, followed by the acetone fraction with inhibitory activity of 22.24 ± 0.41%, 38.05 ± 0.68%, and 46.62 ± 0.32% at increasing concentrations of 50, 200, and 350 ppm, respectively. The acetone-methanol (1:4) mixture exhibited the lowest activity at 8.00 ± 0.35% for 50 ppm, and 0.61 ± 0.27% for 200 ppm. However, no inhibitory activity was demonstrated in any of the fractions of Basella alba against α-glucosidase. Total flavonoid content analysis revealed that all fractions have the same flavonoid content. Basella alba may serve an essential role in managing the risk of diabetes mellitus and is recommended for consumption on a regular basis....
Bioactive components from cucamelon (Melothria scabra) with potential α-amylase and α-glucosidase inhibitory activity
(2022-07)
Cucamelons (Melothria scabra) are found growing in the wild in the Philippines. The lack of available literature regarding the plant species prompted to conduct of this study. This study aimed to extract and fractionate bioactive components from cucamelon with potential α-amylase and α-glucosidase inhibitory activity. Cucamelon fruit samples were obtained from Brgy. Pagsanga-an Pavia, Iloilo, extracted with methanol, fractionated using silica column chromatography, and assayed for α-amylase and α-glucosidase inhibitory activity. Results show that most but not all of the fractions have α-amylase inhibitory activity. Only four fractions showed α-amylase activity, namely, acetone (8.97 ± 1.54), acetone: methanol, 4:1 (15.54 ± 2.12), acetone: methanol, 3:2 (11.30 ± 2.51), and acetone: methanol, 1:4 (17.94 ± 2.19). Only one fraction had α-glucosidase inhibitory activity, which is acetone: methanol, 3:2 (3.59 ± 1.10). Cucamelon has a low inhibitory activity for α-amylase and α-glucosidase, enzyme markers of diabetes, which can be compensated by increasing the daily intake. It is recommended that unripe cucamelon fruits can be eaten regularly as salads, or pickled to help prevent the risk of developing diabetes....
Potential α-amylase and α-glucosidase inhibitory activities of bioactive fractions from calamansi (Citrofortunella microcarpa) peels
(2022-06)
Calamansi fruits are commonly used in the Philippines and the peels are discarded as wastes. Epicarpium of calamansi peels were selected for analysis in this study in order to determine their potential in vitro inhibition ...