In vitro evaluation of the efficacy of the various preparations, concentrations, and post-exposure observation of garlic (Allium sativum) in the development of roundworm (Ascaridia galli) eggs

Abstract

The study was conducted at CPU- CARES, Research and Developmental Learning Building, Jaro, Iloilo City from April 1 to 30, 2021. This was done to evaluate the efficacy of the various preparations, concentrations, and post- exposure observation of garlic in the development of Ascaridia galli eggs. Experimental treatments were composed of different concentrations in different processing method. Goodwin’s solution and Albendazole served as negative and positive control respectively. The data were collected during the 10th, 14th and 21st day of post- exposure. The results showed that the 10th day of post- exposure time the 200 to 500 mg/ml pure juice and 400 and 500 mg/ml of aqueous extract inhibited 100% of egg development. The same trend happened to 500 mg/ml vacuum freeze- dried and 400 and 500 mg/ml dehydrated from the 14th day of post- exposure time. It was found out that various preparations at 100 mg/ml had a comparable result to positive control. Furthermore, eggs exposed to Goodwin’s solution have the lowest percentage of undeveloped eggs from the 10th to 21st day of post- exposure. Based on these results, it is concluded that garlic at different level of concentrations (100, 200, 300, 400, and 500 mg/ ml) inhibited the larval development of A. galli eggs. Garlic exhibits ovicidal effects against A. galli eggs regardless of the methods of preparation (pure juice, aqueous extract, vacuum freeze-dried, and dehydrated) and post-exposure observation.