Formulation and evaluation of antibacterial properties of garlic (Allium sativum) with lavender (Lavandula angustifolia) ointment against Staphylococcus aureus in Philippine native chicken

Abstract

Staphylococcus aureus It is the main source of skin and soft tissue infections, which result in cellulitis, furuncles, abscesses, and mortality. Garlic ointment mixed with lavender oil has been produced as a safer and less expensive alternative to synthetic antibiotic ointment. The study was conducted to formulate and evaluate the antimicrobial activity of Garlic (Allium sativum) with lavender (Lavandula angustifolia) essential oil; assess the Minimum inhibitory Concentration and Zone of Inhibition; and evaluate the Potency Level of Garlic with Lavender Ointment. This research involved a total 30 Petri plates, 100pl Staphylococcus aureus bacteria, 500pl mupirocin at higher dose, 250pl mupirocin at lower dose, 500pl VCO, 500pl distilled water. The concentration were laid out using a complete randomized design (CRD) and two-factor (CRD). The GEO with LEO assessed through MIC after 24 hours of post-exposure revealed that 100% GEO with LEO concentration has the highest mean percentage (80.65%). In addition, treatments 85% to 100% GEO with LEO concentration had a wider ZOI. Furthermore, after 72 hours of post-exposure. The antibacterial property of GEO with LEO is potent, based on standard potency of 60 to 150 percent. Thus, GEO with LEO can serve as a substitute for regulating the isolation of Staphylococcus aureus from Philippine native chicken.