Determination of the lethal dose concentration (LD50) of standardized rosemary (Rosmarinus officinalis) essential oil among the Philippine native chickens

Abstract

This study was conducted at the Central Philippine University, College of Agriculture, Resources, and Environmental Sciences at the Research and Developmental Learning Building. The study began on November 2023 and ended on January 2024. The study was conducted to determine the lethal dosage concentration (LD50) of standardized Rosemary (Rosmarinus officinalis) essential oil among the Philippine native chickens. The research involved analyzing the efficacy of Rosemary essential oil against Pseudomonas aeruginosa. Additionally, a toxicity assessment was conducted on the essential oil, focusing on high dosage effects on Philippine Native Chickens. Four three-month-old native chickens were used in the study. The liveweight gain after treatment was observed to be higher than before, indicating positive effects. No signs of toxicity were observed in the chickens, and all remained alive and active, demonstrating a favorable survival rate. Fecalysis results indicated the deworming effectiveness of rosemary essential oil in eradicating parasitic eggs. Overall, the findings suggest promising results for the antibacterial properties of rosemary essential oil and its potential benefits for native chicken health.