Isolation and identification of microorganisms from water and sediment samples in Iloilo River, Philippines

Abstract

This study explored how common Pseudomonas species are in the Iloilo River, Philippines, along with their biochemical traits and resistance to antibiotics. Results showed these bacteria were more prevalent during the wet season likely due to increased runoff and nutrients entering the river with the highest concentrations found near urban and wastewater discharge areas. Lab tests confirmed the isolates were indeed Pseudomonas, showing typical features like being oxidase-positive, mobile, and able to grow at 40°C. Interestingly, their varying ability to ferment glucose suggests they’re well-adapted to different environmental conditions. What’s especially concerning is that all the isolates were completely resistant to both ampicillin and novobiocin, hinting at how pollution might be fueling the rise of antibiotic-resistant bacteria. These insights highlight the urgent need for ongoing water quality monitoring, stricter pollution controls, and better public awareness to protect both the river’s health and the people who depend on it.