Anthelmintic activity of Areca catechu Linn. and Dioscora hispida Dennst. freeze dried extracts against Ascaridia galli S. in vitro

Abstract

This study was conducted from February 12 to 14, 2019 at CPU-CARES research laboratory at the Research and Development Learning Center, Jaro, Iloilo City. The study was conducted to evaluate the in vitro anthelmintic activity of freeze dried betel nut and bitter yam extracts against adult A. galli worms. The experimental treatments were composed of 100% betel nut; 100% bitter yam; 50% betel nut and 50% bitter yam; 30% betel nut and 70% bitter yam; 70% betel nut and 30% bitter yam. Each of these experimental treatments had varying concentrations (40, 60, 80 mg/ml). Procured albendazole (commercial dewormer), Goodwin’s solution and distilled water were used as control treatments. These treatments were laid out in a completely randomized block design with three replications with four worms for every treatment in each replicate. The anthelmintic activity of extracts was assessed through percentage paralysis/death for 24- hour relative to the use of vermex at 15 mg/ml. Results of the study revealed that both plant extracts either in combination or solely used were found to be time and concentration dependent. After 24-hour exposure, percentage paralysis/death of A. galli worms treated with plant extracts was comparable to that of A. galli applied with synthetic dewormer used. However, bitter yam was a more efficient anthelmintic alternative in treating ascaridiasis in chickens than betel nut in terms of the length of time of worm paralysis.