Evaluation of the in vitro anthelminthic activity of Tinospora rumphii Boerl, Areca catechu L. and Dioscorea hispida Dennst. extract against Ascaridia galli in Philippine native chicken

Abstract

The study was conducted to evaluate the anthelmintic activity of Tinospora rumphii Boerl, Areca catechu L. and Dioscorea hispida Dennst. against Ascaridia galli at three dose concentration (10mg/ml, 20mg/ml, 30mg/ml). Commercial dewormer and Goodwin’s solution was used as positive and negative control, respectively. The study involved the determination of the percent worm mobility inhibition (%WMI) and percent mortality (%M). The experiment was carried out in 33 petri dishes with 4 worms each. The experimental treatments were laid out in a completely randomized design (CRD) with three replications each. Results revealed that 20mg/ml and 30mg/ml of Makabuhay stem extract (MSE) and Asiatic bitter yam extract (ABYE) and commercial dewormer were equally effective in terms of %WMI after 12, 18 and 24 hours post exposure. All the dose concentrations of betel nut extract had the least %WMI and %M. On the other hand, the commercial dewormer, 20 and 30mg/ml MSE and 30mg/ml ABYE showed the highest effect in terms of % mortality of A. galli after 24 hours post exposure with betel nut extract being the least effective among the treatment plants having (66.67%, 75% and 83.33%). It can be concluded that ABYE and MSE at different concentrations significantly affect the %WMI and %M of A. galli worms. With the 20mg/ml and 30mg/ml.