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Antioxidant activity, total phenolic and flavonoid content of bioactive fractions of Symplocos polyandra leaves

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Date
2022
Author
Pelayo, Edgiel Jan G.
Thesis Adviser
Villalobos, Mizpah C.
Defense Panel Chair
Borlongan, Ilda G.
Defense Panel Member
Japitana, Ma. Mercy ORCID
Panadero, Aira Rose P.
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Abstract
Symplocos polyandra, locally known as balakbakan, is distributed throughout Southeast Asia, especially in the Philippines. The lack of available literature regarding the phytochemistry of this plant species prompted the conduct of testing of its potential antioxidant activity and quantification of its total flavonoid content and phenolic content. Balakbakan leaves were collected, exhaustively extracted with methanol, and fractionated on a silica gel column. 2,2-diphenyl-1-picrylhydrazyI (DPPH) assay showed that the acetone fraction has the highest percent DPPH radical inhibition with 86.41% at 500 ppm compared to 0.5 mM ascorbic acid at 87.61%. The acetone - methanol fraction has the highest ferric reducing capacity with a value of 208.24 μM ascorbic acid equivalents at 500 ppm. The total phenolic content of the different fractions ranged from 21.88 to 29.99 pg/mL catechin equivalents, with hexane - acetone fractions having the highest value. The flavonoid content ranged from 14.28 to 16.64 pg/mL catechin equivalents, with the methanol fraction having the highest value. The compounds responsible for the high antioxidant activity may not be a phenolic or flavonoid. Fractions from Symplocos polyandra are a promising source of bioactive compounds with antioxidant activity. Cytotoxicity test and analyses of alkaloid and chlorophyll content of the leaves is recommended for the leaves to be developed into tea products.
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Abstract only
URI
https://hdl.handle.net/20.500.12852/2415
Suggested Citation
Pelayo, E. J. G. (2022). Antioxidant activity, total phenolic and flavonoid content of bioactive fractions of Symplocos polyandra leaves (Unpublished thesis). Central Philippine University, Jaro, Iloilo City.
Type
Thesis
Subject(s)
Antioxidants--Analysis OCLC - FAST (Faceted Application of Subject Terminology); Antioxidants OCLC - FAST (Faceted Application of Subject Terminology); Phenols OCLC - FAST (Faceted Application of Subject Terminology); Phenols--Analysis OCLC - FAST (Faceted Application of Subject Terminology); Flavonoids OCLC - FAST (Faceted Application of Subject Terminology); Flavonoids--Analysis OCLC - FAST (Faceted Application of Subject Terminology); Leaves OCLC - FAST (Faceted Application of Subject Terminology); Foliar diagnosis OCLC - FAST (Faceted Application of Subject Terminology)
Keywords
Balakbakan; Symplocos polyandra
Department
College of Arts and Sciences
Degree
Bachelor of Science in Chemistry
Shelf Location
Filipiniana Theses 540.72 P361
Physical Description
vii, 44 leaves
Collections
  • Bachelor of Science in Chemistry [11]

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