Collaborative Researcheshttps://hdl.handle.net/20.500.12852/152024-03-29T14:03:11Z2024-03-29T14:03:11ZProduction, feeding and storage of Tetraselmis tetrathele paste by electrolytic flocculationVilla‑Franco, Annie U.Teves, Christine Marie L.Dato‑on, Knessa Louie G.de Jesus-Ayson, Evelyn Grace T.Usero, Roselynde la Peña, Milagros R.https://hdl.handle.net/20.500.12852/27312023-07-12T01:48:13Z2023-02-01T00:00:00ZProduction, feeding and storage of Tetraselmis tetrathele paste by electrolytic flocculation
Villa‑Franco, Annie U.; Teves, Christine Marie L.; Dato‑on, Knessa Louie G.; de Jesus-Ayson, Evelyn Grace T.; Usero, Roselyn; de la Peña, Milagros R.
An innovative microalgae harvesting technique using electroflocculation was carried out at different voltage treatments (4, 7, and 9 V) using a fabricated voltage regulator and compared with harvesting by electroflocculation at 12 V using a car battery to efficiently electroflocculate Tetraselmis tetrathele. This study was conducted to determine the ideal voltage settings that could improve the quality of harvested paste in terms of nutritional value and metal contamination for its later use in aquaculture. The use of 7 V achieved low Pb content producing the best harvest biomass (1.4 kg, 87 min) which is comparable to the harvest biomass (1.7 kg, 68 min) using a 12 V car battery. Storage of T. tetrathele paste is best done in a chiller (2 ± 1 °C) rather than a freezer (-20 ± -4 °C) for ease in reactivation and maintaining higher cell viability for later use as a starter in aquaculture. Storage in a chiller allows T. tetrathele paste to be resuspended after six months and used as a starter culture.
Journal article
2023-02-01T00:00:00ZVibrio load and percentage composition of sucrose-fermenting vibrios in Nile tilapia (Oreochromis niloticus) cultured in brackish water earthen ponds and biocontrol potential of recycled tilapia-conditioned greenwater against population growth of V. parahaemolyticus and non-sucrose-fermenting vibrios in the grow-out culture of white leg shrimp (Litopenaeus vannamei)Pakingking, RolandoPalma, Peterde Jesus-Ayson, Evelyn Grace T.Usero, Roselynhttps://hdl.handle.net/20.500.12852/25132023-03-06T00:19:48Z2022-10-20T00:00:00ZVibrio load and percentage composition of sucrose-fermenting vibrios in Nile tilapia (Oreochromis niloticus) cultured in brackish water earthen ponds and biocontrol potential of recycled tilapia-conditioned greenwater against population growth of V. parahaemolyticus and non-sucrose-fermenting vibrios in the grow-out culture of white leg shrimp (Litopenaeus vannamei)
Pakingking, Rolando; Palma, Peter; de Jesus-Ayson, Evelyn Grace T.; Usero, Roselyn
<i>Vibrio</i> are known opportunistic pathogens of pond-cultured fish and shrimp. This study determined the presumptive <i>Vibrio</i> count (PVC) and percentage composition of “beneficial” sucrose-fermenting vibrios (SFVs) and “pathogenic” non-SFVs in the water, sediment, gills and intestines of healthy tilapia <i>Oreochromis niloticus</i> collected every 2 weeks from Days 30 to 120 post-stocking in brackish water earthen ponds. PVCs in water and sediment ranged from 10<sup>2</sup>–10<sup>4</sup> CFU/ml/CFU/g while in tilapias' gills and intestines, PVCs ranged from 10<sup>3</sup>–10<sup>6</sup> CFU/g and 10<sup>2</sup>–10<sup>6</sup> CFU/g, respectively, with SFVs dominating in water, sediment, gills (≥50%), and intestines (≥80%) of tilapia. Because use of tilapia-conditioned green water (TCGW) for shrimp's grow-out culture has been reported effective against acute hepatopancreatic necrosis disease outbreaks caused by <i>V. parahaemolyticus</i>, PVC, SFVs/non-SFVs (%), and <i>V. parahaemolyticus</i> count (VPC) in the recycled TCGW and <i>Litopenaeus vannamei</i>'s hepatopancreas were also examined from stocking until 105 days of culture (DOC) in earthen ponds. PVCs ranging from 10<sup>2</sup>–10<sup>3</sup> CFU/ml and 10<sup>3</sup>–10<sup>4</sup> CFU/g were noted in water and shrimp's hepatopancreas, respectively. While non-SFVs population in water varied, a downward trend typified by an abrupt 50% reduction was noted in hepatopancreas at DOC 42, and remained or further dropped until DOC 105. VPCs in water likewise varied (undetectable–10<sup>2</sup> CFU/ml) while in hepatopancreas, counts dropped to undetectable level from DOC 70 onwards. Current data illustrate that remarkable shift of SFVs' dominance in the water and concomitantly in shrimp's hepatopancreas, plays a pivotal role in regulating non-SFVs proliferation in shrimp's hepatopancreas via competitive exclusion.
Journal article
2022-10-20T00:00:00ZMedicinal and phytochemical properties of selected herbal plants in Central China and Western Visayas, PhilippinesFernandez, Stella G.Guanzon, Nicolas G. Jr.Li, KeLi, MengZai, Guangyuhttps://hdl.handle.net/20.500.12852/24982023-02-27T04:00:17Z2021-11-01T00:00:00ZMedicinal and phytochemical properties of selected herbal plants in Central China and Western Visayas, Philippines
Fernandez, Stella G.; Guanzon, Nicolas G. Jr.; Li, Ke; Li, Meng; Zai, Guangyu
This investigation was conducted to evaluate the medicinal and phytochemical properties of four selected herbal plants: <em>Andrographis paniculata, Cassia obtusifolia, Leonurus japonicus</em> and <em>Pueraria lobata</em> found in both Central China and Western Visayas, Philippines. Mixed method research design was employed, the quantitative on herbals' descriptions and phytochemical analysis, while qualitative on interview of traditional healers. The herbals were identified and authenticated by the research team. The phytochemical analyses used the methods: Extraction of plant parts, Thin Layer Chromatography, UV identification and High-Performance Liquid Chromatography. The bioactive compounds diterpenoid dehydroangropholide in <em>A. paniculata</em> supports the claim of local healers to treat stomach ache and diabetes; the terpenoid orange cassia and chrysophanol in <em>C. obtusifolia</em> seeds supports the treatment for ulcer, hepatitis or liver associated ailment, ringworm, scabies and psoriasis; the alkaloid leonurine hydrochloride in <em>L. japonicus</em> leaves supports the healing practices in treating menstrual disorders and fever; and the flavonoid puerarin in <em>P. lobata</em> tubers supports the claim of traditional healers in regulating menstruation, diuresis, fever and promotes blood circulation. Most healing practices in both study areas have scientific explanations.
Journal article
2021-11-01T00:00:00ZFood and organizations: The relationship of organizational support and attitude towards office gastrodiplomacy among employees of a public organizationBajar, Jayson Troy F.Dela Peña, Renia F.https://hdl.handle.net/20.500.12852/24432023-02-01T07:00:17Z2022-12-20T00:00:00ZFood and organizations: The relationship of organizational support and attitude towards office gastrodiplomacy among employees of a public organization
Bajar, Jayson Troy F.; Dela Peña, Renia F.
Office gastrodiplomacy refers to how food shapes and influences our social relations within organizations, usually in positive ways. Although already a pervasive practice, it is only recently that this concept has received wide attention from industry practitioners and researchers in the behavioral sciences. This emerging topic traces its origins to socioanthropological studies applied to modern work environments like office settings. To expound scholarship in this field, particularly in the local parlance, this study aims to present novel findings on how organizational support, in aspects involving staff development, resources, and emotional support, affects attitude towards office gastrodiplomacy. A total of 327 respondents participated in a one-shot survey administered from September to December 2019 at the 25 offices of a public organization in the Philippines. Employing a descriptiverelational design, descriptive findings revealed that employees indicated being ‘fairly supported’ by their offices, while a higher proportion of respondents stated that they generally have a ‘favorable attitude’ towards office gastrodiplomacy. Relational statistics, however, generated a ‘not significant relationship’ between the two variables as a whole and across the three components. The researchers argue that it is likely that such a finding is a result of how the respondents only received organizational support in ‘fair’ terms. As such, results may somewhat differ if a ‘high’ favorable attitude to office gastrodiplomacy is complemented with ‘high’ organizational support. It is then proposed that a similar design may be replicated among employees in private organizations who may offer a different perspective, especially as regards aspects of organizational support.
Conference paper
2022-12-20T00:00:00Z